Control:Article Title: Witnessing Phenotypic and Molecular Evolution in the Fruit Fly.
Article Snippet: .. Learning Objectives Between the laboratory exercise and the associated lectures, the students will: & Set up control white-eyed fruit fly populations and experimental ones with a “mutant” red-eyed male added & Directly observe greater mating success of the red-eyed male & Identify that, as a result of the red-eyed males greater mating success, the red eye variant (allele) is more abundant in the next generations: a personally observed case of “evolution by natural selection” & Understand X-linkage, in part by seeing that none of the F1 offspring males have red eyes even though most of the females do & Learn about and apply polymerase chain reaction (PCR) and gel electrophoresis for the purpose of understanding an evolutionary process & Learn about physical linkage of segments on the same chromosome & Demonstrate the molecular evolution concept of “hitchhiking” of alleles at other genes along with the spread of an advantageous mutation Materials & D. simulans w138a (white), UC San Diego Drosophila Species Stock Center, stock number 14021-0251.138a & D. simulans w195 (white), UC San Diego Drosophila Species Stock Center, stock number 14021-0251.195 & D. simulans C167.4 (wild type), UC SanDiegoDrosophila Species Stock Center, stock number 14021-0251.199 & Carolina Biological Supply: Drosophila Kit (FlyNap®, 72 vials, 72 plugs, media, labels, transfer cards): 173052 & (Optional but strongly preferred) CO2 anesthetization facilities for Drosophila & “Squishing buffer” and proteinase-K for DNA isolation (protocol in Appendix) & Equipment and reagents for polymerase chain reaction including oligonucleotides for “Near” and “Far”markers in the Appendix & Equipment and reagents for gel electrophoresis & Magnifying glasses & Sharpie markers & PowerPoint lectures (available online) and projector (optional) Material Notes Because of the availability of multiple wholegenome sequences (Begun et al. 2007) that were leveraged in its design, this laboratory exercise uses the species D. simulans rather than its sister species Drosophila melanogaster. ..
Mutagenesis:Article Title: Witnessing Phenotypic and Molecular Evolution in the Fruit Fly.
Article Snippet: .. Learning Objectives Between the laboratory exercise and the associated lectures, the students will: & Set up control white-eyed fruit fly populations and experimental ones with a “mutant” red-eyed male added & Directly observe greater mating success of the red-eyed male & Identify that, as a result of the red-eyed males greater mating success, the red eye variant (allele) is more abundant in the next generations: a personally observed case of “evolution by natural selection” & Understand X-linkage, in part by seeing that none of the F1 offspring males have red eyes even though most of the females do & Learn about and apply polymerase chain reaction (PCR) and gel electrophoresis for the purpose of understanding an evolutionary process & Learn about physical linkage of segments on the same chromosome & Demonstrate the molecular evolution concept of “hitchhiking” of alleles at other genes along with the spread of an advantageous mutation Materials & D. simulans w138a (white), UC San Diego Drosophila Species Stock Center, stock number 14021-0251.138a & D. simulans w195 (white), UC San Diego Drosophila Species Stock Center, stock number 14021-0251.195 & D. simulans C167.4 (wild type), UC SanDiegoDrosophila Species Stock Center, stock number 14021-0251.199 & Carolina Biological Supply: Drosophila Kit (FlyNap®, 72 vials, 72 plugs, media, labels, transfer cards): 173052 & (Optional but strongly preferred) CO2 anesthetization facilities for Drosophila & “Squishing buffer” and proteinase-K for DNA isolation (protocol in Appendix) & Equipment and reagents for polymerase chain reaction including oligonucleotides for “Near” and “Far”markers in the Appendix & Equipment and reagents for gel electrophoresis & Magnifying glasses & Sharpie markers & PowerPoint lectures (available online) and projector (optional) Material Notes Because of the availability of multiple wholegenome sequences (Begun et al. 2007) that were leveraged in its design, this laboratory exercise uses the species D. simulans rather than its sister species Drosophila melanogaster. ..
Variant Assay:Article Title: Witnessing Phenotypic and Molecular Evolution in the Fruit Fly.
Article Snippet: .. Learning Objectives Between the laboratory exercise and the associated lectures, the students will: & Set up control white-eyed fruit fly populations and experimental ones with a “mutant” red-eyed male added & Directly observe greater mating success of the red-eyed male & Identify that, as a result of the red-eyed males greater mating success, the red eye variant (allele) is more abundant in the next generations: a personally observed case of “evolution by natural selection” & Understand X-linkage, in part by seeing that none of the F1 offspring males have red eyes even though most of the females do & Learn about and apply polymerase chain reaction (PCR) and gel electrophoresis for the purpose of understanding an evolutionary process & Learn about physical linkage of segments on the same chromosome & Demonstrate the molecular evolution concept of “hitchhiking” of alleles at other genes along with the spread of an advantageous mutation Materials & D. simulans w138a (white), UC San Diego Drosophila Species Stock Center, stock number 14021-0251.138a & D. simulans w195 (white), UC San Diego Drosophila Species Stock Center, stock number 14021-0251.195 & D. simulans C167.4 (wild type), UC SanDiegoDrosophila Species Stock Center, stock number 14021-0251.199 & Carolina Biological Supply: Drosophila Kit (FlyNap®, 72 vials, 72 plugs, media, labels, transfer cards): 173052 & (Optional but strongly preferred) CO2 anesthetization facilities for Drosophila & “Squishing buffer” and proteinase-K for DNA isolation (protocol in Appendix) & Equipment and reagents for polymerase chain reaction including oligonucleotides for “Near” and “Far”markers in the Appendix & Equipment and reagents for gel electrophoresis & Magnifying glasses & Sharpie markers & PowerPoint lectures (available online) and projector (optional) Material Notes Because of the availability of multiple wholegenome sequences (Begun et al. 2007) that were leveraged in its design, this laboratory exercise uses the species D. simulans rather than its sister species Drosophila melanogaster. ..
Selection:Article Title: Witnessing Phenotypic and Molecular Evolution in the Fruit Fly.
Article Snippet: .. Learning Objectives Between the laboratory exercise and the associated lectures, the students will: & Set up control white-eyed fruit fly populations and experimental ones with a “mutant” red-eyed male added & Directly observe greater mating success of the red-eyed male & Identify that, as a result of the red-eyed males greater mating success, the red eye variant (allele) is more abundant in the next generations: a personally observed case of “evolution by natural selection” & Understand X-linkage, in part by seeing that none of the F1 offspring males have red eyes even though most of the females do & Learn about and apply polymerase chain reaction (PCR) and gel electrophoresis for the purpose of understanding an evolutionary process & Learn about physical linkage of segments on the same chromosome & Demonstrate the molecular evolution concept of “hitchhiking” of alleles at other genes along with the spread of an advantageous mutation Materials & D. simulans w138a (white), UC San Diego Drosophila Species Stock Center, stock number 14021-0251.138a & D. simulans w195 (white), UC San Diego Drosophila Species Stock Center, stock number 14021-0251.195 & D. simulans C167.4 (wild type), UC SanDiegoDrosophila Species Stock Center, stock number 14021-0251.199 & Carolina Biological Supply: Drosophila Kit (FlyNap®, 72 vials, 72 plugs, media, labels, transfer cards): 173052 & (Optional but strongly preferred) CO2 anesthetization facilities for Drosophila & “Squishing buffer” and proteinase-K for DNA isolation (protocol in Appendix) & Equipment and reagents for polymerase chain reaction including oligonucleotides for “Near” and “Far”markers in the Appendix & Equipment and reagents for gel electrophoresis & Magnifying glasses & Sharpie markers & PowerPoint lectures (available online) and projector (optional) Material Notes Because of the availability of multiple wholegenome sequences (Begun et al. 2007) that were leveraged in its design, this laboratory exercise uses the species D. simulans rather than its sister species Drosophila melanogaster. ..
Polymerase Chain Reaction:Article Title: Witnessing Phenotypic and Molecular Evolution in the Fruit Fly.
Article Snippet: .. Learning Objectives Between the laboratory exercise and the associated lectures, the students will: & Set up control white-eyed fruit fly populations and experimental ones with a “mutant” red-eyed male added & Directly observe greater mating success of the red-eyed male & Identify that, as a result of the red-eyed males greater mating success, the red eye variant (allele) is more abundant in the next generations: a personally observed case of “evolution by natural selection” & Understand X-linkage, in part by seeing that none of the F1 offspring males have red eyes even though most of the females do & Learn about and apply polymerase chain reaction (PCR) and gel electrophoresis for the purpose of understanding an evolutionary process & Learn about physical linkage of segments on the same chromosome & Demonstrate the molecular evolution concept of “hitchhiking” of alleles at other genes along with the spread of an advantageous mutation Materials & D. simulans w138a (white), UC San Diego Drosophila Species Stock Center, stock number 14021-0251.138a & D. simulans w195 (white), UC San Diego Drosophila Species Stock Center, stock number 14021-0251.195 & D. simulans C167.4 (wild type), UC SanDiegoDrosophila Species Stock Center, stock number 14021-0251.199 & Carolina Biological Supply: Drosophila Kit (FlyNap®, 72 vials, 72 plugs, media, labels, transfer cards): 173052 & (Optional but strongly preferred) CO2 anesthetization facilities for Drosophila & “Squishing buffer” and proteinase-K for DNA isolation (protocol in Appendix) & Equipment and reagents for polymerase chain reaction including oligonucleotides for “Near” and “Far”markers in the Appendix & Equipment and reagents for gel electrophoresis & Magnifying glasses & Sharpie markers & PowerPoint lectures (available online) and projector (optional) Material Notes Because of the availability of multiple wholegenome sequences (Begun et al. 2007) that were leveraged in its design, this laboratory exercise uses the species D. simulans rather than its sister species Drosophila melanogaster. ..
Nucleic Acid Electrophoresis:Article Title: Witnessing Phenotypic and Molecular Evolution in the Fruit Fly.
Article Snippet: .. Learning Objectives Between the laboratory exercise and the associated lectures, the students will: & Set up control white-eyed fruit fly populations and experimental ones with a “mutant” red-eyed male added & Directly observe greater mating success of the red-eyed male & Identify that, as a result of the red-eyed males greater mating success, the red eye variant (allele) is more abundant in the next generations: a personally observed case of “evolution by natural selection” & Understand X-linkage, in part by seeing that none of the F1 offspring males have red eyes even though most of the females do & Learn about and apply polymerase chain reaction (PCR) and gel electrophoresis for the purpose of understanding an evolutionary process & Learn about physical linkage of segments on the same chromosome & Demonstrate the molecular evolution concept of “hitchhiking” of alleles at other genes along with the spread of an advantageous mutation Materials & D. simulans w138a (white), UC San Diego Drosophila Species Stock Center, stock number 14021-0251.138a & D. simulans w195 (white), UC San Diego Drosophila Species Stock Center, stock number 14021-0251.195 & D. simulans C167.4 (wild type), UC SanDiegoDrosophila Species Stock Center, stock number 14021-0251.199 & Carolina Biological Supply: Drosophila Kit (FlyNap®, 72 vials, 72 plugs, media, labels, transfer cards): 173052 & (Optional but strongly preferred) CO2 anesthetization facilities for Drosophila & “Squishing buffer” and proteinase-K for DNA isolation (protocol in Appendix) & Equipment and reagents for polymerase chain reaction including oligonucleotides for “Near” and “Far”markers in the Appendix & Equipment and reagents for gel electrophoresis & Magnifying glasses & Sharpie markers & PowerPoint lectures (available online) and projector (optional) Material Notes Because of the availability of multiple wholegenome sequences (Begun et al. 2007) that were leveraged in its design, this laboratory exercise uses the species D. simulans rather than its sister species Drosophila melanogaster. ..
DNA Extraction:Article Title: Witnessing Phenotypic and Molecular Evolution in the Fruit Fly.
Article Snippet: .. Learning Objectives Between the laboratory exercise and the associated lectures, the students will: & Set up control white-eyed fruit fly populations and experimental ones with a “mutant” red-eyed male added & Directly observe greater mating success of the red-eyed male & Identify that, as a result of the red-eyed males greater mating success, the red eye variant (allele) is more abundant in the next generations: a personally observed case of “evolution by natural selection” & Understand X-linkage, in part by seeing that none of the F1 offspring males have red eyes even though most of the females do & Learn about and apply polymerase chain reaction (PCR) and gel electrophoresis for the purpose of understanding an evolutionary process & Learn about physical linkage of segments on the same chromosome & Demonstrate the molecular evolution concept of “hitchhiking” of alleles at other genes along with the spread of an advantageous mutation Materials & D. simulans w138a (white), UC San Diego Drosophila Species Stock Center, stock number 14021-0251.138a & D. simulans w195 (white), UC San Diego Drosophila Species Stock Center, stock number 14021-0251.195 & D. simulans C167.4 (wild type), UC SanDiegoDrosophila Species Stock Center, stock number 14021-0251.199 & Carolina Biological Supply: Drosophila Kit (FlyNap®, 72 vials, 72 plugs, media, labels, transfer cards): 173052 & (Optional but strongly preferred) CO2 anesthetization facilities for Drosophila & “Squishing buffer” and proteinase-K for DNA isolation (protocol in Appendix) & Equipment and reagents for polymerase chain reaction including oligonucleotides for “Near” and “Far”markers in the Appendix & Equipment and reagents for gel electrophoresis & Magnifying glasses & Sharpie markers & PowerPoint lectures (available online) and projector (optional) Material Notes Because of the availability of multiple wholegenome sequences (Begun et al. 2007) that were leveraged in its design, this laboratory exercise uses the species D. simulans rather than its sister species Drosophila melanogaster. ..
|